Fig. 5: Promoter transcription initiation frequency (TIF) of 54 forward engineered sigma factor 70 (σ⁷⁰) promoters in function of their predicted class.

Values are corrected fluorescence measurements (fluorescence_C), logarithmic transformed and normalized between 0 and 1. Data are represented as mean values +/− the standard deviation derived from eight biological replicates. The linear regression line (R² = 0.876) is depicted together with the 95% confidence interval (shaded area) and the 50% prediction interval (red dashed lines). Source data are provided as a Source Data file.