Fig. 3: Screen of neural genes that could rescue NMJ defects and larvae mobility. | Nature Communications

Fig. 3: Screen of neural genes that could rescue NMJ defects and larvae mobility.

From: Defective minor spliceosomes induce SMA-associated phenotypes through sensitive intron-containing neural genes in Drosophila

Fig. 3

a Left, immunostaining of neuromuscular connections in transgenic strains driven by elav-GAL4 in the SmnΔ/Δ background. NMJ boutons are counted and the additional transgenic strains are shown in Supplementary Fig. 5a. Right, quantitation of boutons in each strain (SmnΔ/Δ; + Smn: p = 8.3e-5; SmnΔ/Δ; + Zmynd10: p = 0.00021; SmnΔ/Δ; + CG10171: p = 8.3e−5; SmnΔ/Δ; + Pcyt2: p = 0.00017; SmnΔ/Δ; + Fas3: p = 0.00025). b Left, larval locomotion of transgenic strains (SmnΔ/Δ; + Smn: p = 8.1e−6; SmnΔ/Δ; + Zmynd10: p = 0.0001; SmnΔ/Δ; + Pcyt2: p = 9.3e−5; SmnΔ/Δ; + Fas3: p = 0.00442). Additional transgenic strains are shown in Supplementary Fig. 5d. Right, quantitation of path length of larval locomotion. Data represent the mean ± SD from five representatives of each strain. **p < 0.01, ***p < 0.001. P values were calculated using two-sided t-test. Source data are provided as a Source Data file.

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