Fig. 5: Association of new minS-Is with minor-spliceosomal components. | Nature Communications

Fig. 5: Association of new minS-Is with minor-spliceosomal components.

From: Defective minor spliceosomes induce SMA-associated phenotypes through sensitive intron-containing neural genes in Drosophila

Fig. 5

a Predicted stability of RNA duplexes formed by the 5′SS of minS-Is and U11 snRNA. Potential base-pairs and free energy are calculated by RNAduplex; both Drosophila and human U11 snRNAs are used for duplex analyses. n = 15 (U12 intron); n = 48 (new minS-Is); n = 105 (single sensitive 5′SSs); n = 150 (single sensitive 3′SSs). b Dm65K is a specific component of the minor spliceosome. The FTH-Dm65K strain constructed using the CRISPR/Cas9 system is validated by PCR, sequencing, and western blot (upper). FTH-Dm65K enriches minor snRNAs and U12-type introns, but not major snRNA or U2-type introns (lower). Co-purifications were performed using lysate from the WT and FTH-Dm65K Drosophila strains. c Association of new minS-Is with Dm65K. FTH-Dm65K enriches pre-mRNA of most new minS-Is that contain 5′SS predicted to form a stable duplex with U11 snRNA. minS-Is (red line) and free energy (ΔG) are indicated. In (a) boxplots, the middle line show data median, the lower and upper hinges correspond to the 25th and 75th percentiles, the upper whisker extends from the hinge to the largest value no further than 1.5  × IQR from the hinge (where IQR is the interquartile range) and the lower whisker extends from the hinge to the smallest value at most 1.5 × IQR of the hinge. Source data are provided as a Source Data file.

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