Fig. 6: Alternative selection by the major and minor spliceosomes. | Nature Communications

Fig. 6: Alternative selection by the major and minor spliceosomes.

From: Defective minor spliceosomes induce SMA-associated phenotypes through sensitive intron-containing neural genes in Drosophila

Fig. 6

a Alternative splicing events that are involved with minor spliceosome sensitive SSs. Upper, alternative 5′SS events identified from three categories of minS-5′SSs; lower, alternative 3′SS events identified from three categories of minS-3′SSs. b Upper, schematics of splice sites in the minS-SS-involved AS events. Lower, splicing of introns using the alternative SSs and common SSs were increased in the minor-snRNA deletion strains. c Common SSs can be recognized by both the major and minor spliceosomes. Five sets of primers were used to amplify various pre-mRNAs and mRNAs. d Pre-mRNAs but not mRNAs of the minor spliceosome-sensitive genes are enriched by minor-specific Dm65K. e Pre-mRNAs of the genetically identified three genes that rescue SMA-associated phenotypes are enriched by Dm65K. FTH-Dm65K and the WT strains were used. Red arrows and lines: minS-SSs and minS-Is; black arrows and lines: the major spliceosome recognized SSs and introns; blue lines: mixture of the minor and major spliceosomes recognized introns. Source data are provided as a Source Data file.

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