Fig. 6: MOL2 and MOL5/6 change their transcriptional profile following traumatic spinal cord injury.
a, b Uniform Manifold Approximation and Projection (UMAP) plots showing 1967 Sox10::Cre-GFP-positive cells from laminectomy control, injury site, and Wallerian degeneration regions contributing to the clusters (a) and cell lineage composition (b), as determined by graph-based clustering (Seurat)27,28 and integration of labels with the Marques et al. scRNA-Seq dataset5,17. c, d Frequency distribution of the OL lineage cells from the laminectomy control and injury site (c) and Wallerian degeneration regions and injury site (d) forming the clusters. e, f 2D Volcano plots showing the differentially expressed genes (e) and biological processes (f) in the OL lineage cells from the injury site compared to laminectomy control and Wallerian degeneration regions. Gene symbols are colored according to the clusters that are enriched at, while the corresponding dot is colored according to the p value. The OL lineage was sorted from Sox10::Cre-GFP mouse line 3 months following spinal cord injury. CTRL laminectomy control, IS injury site, WD Wallerian degeneration. OPC oligodendrocyte progenitor cell, COP committed OPC, NFOL newly formed oligodendrocyte, MFOL myelin forming oligodendrocyte, MOL mature oligodendrocyte. Source data are provided as a Source Data file.
