Fig. 2: Protein insertions form an extended interface between γ-TuSC spokes. | Nature Communications

Fig. 2: Protein insertions form an extended interface between γ-TuSC spokes.

From: The cryo-EM structure of a γ-TuSC elucidates architecture and regulation of minimal microtubule nucleation systems

Fig. 2

a Zoom on the interface between the Spc97 and Spc98 GRIP1 domains as indicated in the overview. The Spc97 interface is represented as surface, color-coded according to hydrophobicity (brown: hydrophobic; purple: hydrophilic). Spc98 is represented as ribbon, with amino acid side chains mediating the hydrophobic interaction shown and depicted in red. b Interface between the human GCP2 (left) and GCP3 (left) GRIP1 domains. Complementary charged patches on the two surfaces mediate the interaction. Surface representation of the atomic model, colored according to charge (red: negative, blue: positive, and white: no charge). c Zoom on the fungi-specific extended interface between the γ-TuSC spokes as indicated in the overview. The atomic model (white) is shown superposed to the cryo-EM density (transparent). Fungi-specific loops mediating the interaction are colored as in Fig. 1a. d Zoom on the interface between Spc97 and the Spc98 insertion, as indicated in c. Representation as in a. e Zoom on the interface between Spc98 and the γ-tubulin insertion, as indicated in c. Representation as in a, but with Spc98 in surface representation.

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