Fig. 5: The impact of abGCs on MCs odor coding gradually decreases with age.
From: Young adult-born neurons improve odor coding by mitral cells
a The timeline of the experiment. b Left: 2P micrographs of MCs before and after CNO administration, 8, 12, and 16 WPI. Right: calcium transients before (blue) and after (red) CNO injection, from three neurons (marked on the adjacent micrographs). All details are as in Fig. 3b. c Cumulative distribution of the proportion of MCs responding to 0–11 odors, across conditions (8 WPI: n = 113 cells, p << 0.0001; 12 WPI: n = 101 cells, p < 0.05, 16 WPI: n = 104 cells, p = 0.96, Kolmogorov–Smirnov tests, two-tailed for 8 WPI, one-tailed for 12, 16 WPI). d Traces of the average ± SEM calcium responses to odor stimulation before and after CNO at the three age groups. e All responding cell-odor pairs before and after CNO at the three age groups. Red dots are significantly away from the diagonal. The proportions of cell-odor pairs showing significant difference before vs. after were significantly different between the different sessions (n = 1666 cell-odor pairs in total, p << 0.0001, Chi squared test for equality of proportions). f Quantitative analysis of the difference in MCs responsiveness along age (F2,182 = 17.96, p << 0.0001, one-way ANOVA for repeated measures; 8 WPI vs. 12 WPI: n = 101 MCs, p < .01; 8 WPI vs. 16 WPI: n = 103 MCs, p << 0.0001; 12 WPI vs. 16 WPI: n = 92 MCs, p < 0.01, Tukey–Kramer post hoc tests). g Quantitative analysis of the difference in response magnitude due to CNO along age (excited responses: n = 1007 cell-odor pairs in total, F2,1004 = 5.75, p < 0.01, one-way ANOVA; 8 WPI vs. 12 WPI: n = 655, p < 0.01; 8WPI vs. 16WPI: n = 707, p < 0.01; 12 WPI vs. 16 WPI: n = 652, p = 0.39; Suppressed responses: n = 504 cell-odor pairs, F2,591 = 38.11, p << 0.0001, one-way ANOVA; 8 WPI vs. 12 WPI, n = 436, p < 0.001; 8 WPI vs. 16 WPI, n = 396, p << 0.0001; 12 WPI vs. 16 WPI, n = 248, p < 0.001; all pairwise comparisons were conducted with Tukey–Kramer post hoc tests). 8 WPI condition: N = 5 mice; 12 WPI condition: N = 4 mice; 16 WPI condition: N = 4 mice, for all comparisons described in this figure. h DCIs at the three age groups. (F2,130 = 125, p << 0.0001, one-way ANOVA for repeated measures; 8 WPI vs. 12 WPI; p < 0.001, 8 WPI vs. 16 WPI and 12 WPI vs. 16 WPI; p << 0.0001, Tukey–Kramer post hoc tests). Statistical tests are two-sided and error bars are SEMs, unless otherwise stated.
