Fig. 3: Gene expression analysis of Tp63-expressing epithelial stem cells before and after skin transplantation. | Nature Communications

Fig. 3: Gene expression analysis of Tp63-expressing epithelial stem cells before and after skin transplantation.

From: Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence

Fig. 3

A RNA-Seq analysis was performed on epithelial stem cells cultured from whisker hair follicle (WH), vagina (Va), thymus (Thy), bladder (dome) (BL), and trachea (Tr) before they were transplanted into a newborn mouse skin microenvironment (1st round) and after they were recovered from the transplants (2nd round recovered). Cells were FACS sorted on EGFP expression before total RNA was extracted. a Principal component analysis (PCA) of the full transcriptomes. b Expression clustering analysis. c Venn diagram showing the number of up- and downregulated genes, fold-change >2, adjusted p value < 0.05 for each tissue in comparison with hair follicle adjusted p value < 0.05. d Heat map for the expression of some representative genes of different pathways important for hair follicle development and signaling. e Dot plot showing the expression of several genes of the Wnt and Notch signaling pathways in epithelial cells cultured before and after transplantation (1st and 2nd rounds of culture), determined by qPCR analysis. Housekeeping genes were used as normalizer (TBP, SDHA, and Tubb). Mean of 3–9 technical replicates with individual data represented by empty circles. Rec: recovered cells, 2nd round of culture.

Back to article page