Fig. 4: Platelet spreading and haptotactic migration preserve vascular integrity in inflammation.
From: Vascular surveillance by haptotactic blood platelets in inflammation and infection
a, b Analysis of Arpc2+/+ and Arpc2−/− platelet morphology in the inflamed microvasculature. a Cremaster whole mount of adherent platelets. Asterisk: lamellipodium; arrows: filopodia. b Filopodia per cell and solidity, Arpc2+/+: n = 28 cells, Arpc2−/−: n = 30 cells in three mice per group, t tests. Scale bar = 5 µm. c Motility patterns of transfused Arpc2+/+ and Arpc2−/− platelets. Quantification of patterns (n = 21 vessels from three mice), Mann–Whitney. d Distribution and MFI profile of vessel-deposited fibrinogen in relation to VE-Cadherin+ endothelial junctions. Scale bar = 5 µm. e Localization of Arpc2+/+ and Arpc2−/− platelets in relation to VE-Cadherin+ endothelial junctions. (Left) Exemplary whole mounts, arrows indicate platelets adherent to junctions, asterisks highlight platelets distant from junctions and (right) platelet distance from junctions, violin plot, Arpc2+/+: n = 135, Arpc2−/−: n = 144 cells from three mice per group, t test. Scale bar = 10 µm. f Platelet recruitment to α-SMA low regions in Arpc2+/+ and Arpc2−/− mice. (Left) Whole mount and (right) percentage of α-SMA low areas positive for platelets, Arpc2+/+: n = 16, Arpc2−/−: n = 16 vessels from three mice per group, t test. Scale bar = 5 µm. White arrows: platelets. g Bleeding assessment in the inflamed cremaster microvasculature of Arpc2+/+ and Arpc2−/− mice, representative whole mounts stained for erythrocytes (TER119) and vasculature (CD31) and quantification of extravascular TER119 signal, Arpc2+/+: n = 8, Arpc2−/−: n = 5 mice per group, t test. Scale bar = 50 µm. Error bars = s.e.m. All statistical tests are two-sided. Source data are provided as a Source Data file.
