Fig. 7: Migrating platelets protect from bacterial dissemination in MRSA pneumonia.
From: Vascular surveillance by haptotactic blood platelets in inflammation and infection
a Platelet recruitment and clearance in sterile and MRSA biofilms in vitro, n = 5 experiments, Mann–Whitney. Scale bar = 10 µm. b In vitro effect of Arp2/3 inhibition with CK666 compared to control CK689 in platelet-mediated MRSA biofilm clearance. (Left) Percentage of MRSA associated with platelets, n = 7 experiments, t test, and (right) 3D reconstruction of platelets interacting with MRSA. Scale bar = 5 µm. c Scanning electron microscopy of platelet surface-bound MRSA. Inset: bundled bacteria. Scale bar = 1 µm. d–i MRSA lung infection model of Arpc2+/+ and −/− mice, (d) (left) mice were intranasally inoculated, and (right) exemplary micrograph of platelet–MRSA interaction in the lung of Arpc2+/+ mice. Asterisks: lamellipodium; arrows: bundled bacteria. e Micrographs of platelet–MRSA localization and f percentage of MRSA associated with platelets in non-abscessing infected lung tissue (Arpc2+/+: n = 7, Arpc2−/−: n = 9 mice per group). g Percentage of mice with bacteremia was assessed 24 h (n = 16) and 48 h p.i. (Arpc2+/+: n = 11, Arpc2−/−: n = 12 mice per group). h MRSA CFUs recovered from blood at 48 h p.i. (i), percentage of mice with MRSA in the kidney and spleen (Arpc2+/+: n = 15, Arpc2−/−: n = 16 mice). d, h Mann–Whitney and g, i χ2. Scale bars = 5 µm. Error bars = s.e.m. All statistical tests are two-sided. Source data are provided as a Source data file.
