Fig. 2: LIC1 interaction with EF-hand-containing dynein–dynactin adaptors.
From: A tunable LIC1-adaptor interaction modulates dynein activity in a cargo-specific manner
a Domain organization of EF-hand-containing adaptors, highlighting the EF-hand pair of CRACR2a that was co-crystallized with LIC1433–458. b–d ITC titrations of EF-hand pairs from human CRACR2a, FIP3 and NIN into MBP-LIC1FL or LIC1433–458 and in the presence of 5 mM CaCl2 or 5 mM EGTA (as indicated). ITC data shown are representative of n = 2 independent experiments, with similar results. Listed for each titration are the experimental conditions and fitting parameters, including the dissociation constant (KD) and binding stoichiometry (N) derived from fitting to a binding isotherm (black line). The KD and N values are given as mean ± s.d. of the fits. Open symbols correspond to a control titration into buffer.
