Fig. 2: Longitudinal cellular immune profiling in parents and children.

a Major immune cell populations in PBMC at day 12, 37, and 88 in parents (solid line) and children (broken line) (A1 (closed circles), A2 (closed squares), C1 (open circles), C2 (open squares), C3 (open triangles)). b tSNE dimensionality reduction of immune cell populations in all PBMC samples across the three-time points. The tSNE plot was generated from a concatenated file containing 300,000 events (20,000 randomly selected live single cells per patient per time point). c. Frequency of monocyte subpopulations in PBMC from parents and children. d Frequency of CD8 T cell naive, effector, and memory subpopulations in PBMC. e Frequency of PD1 expressing CD8 T cells over time. f Frequency of CD4 T cell naive, effector, and memory subpopulations in PBMC. g tSNE dimensionality reduction of whole blood samples. The tSNE plot was generated from a concatenated file containing 300,000 events (20,000 randomly selected live single cells per patient per time point). Coloring depicts SSC and CD16 expression in tSNE islands. Granulocyte populations (neutrophils and eosinophils) are expressed as the proportion of leukocytes. h Frequency of low-density CD16⁺SSC^hi neutrophils (CD14⁺ and CD14⁻) in PBMC fraction at day 88. i Plasma cytokine concentration of three detectable cytokines, RANTES (blue), MCP-1 (purple), and IL-8 (green) in children and parents at day 12 and 37.