Fig. 4: TPF imaging through an intact mouse skull.
From: Laser scanning reflection-matrix microscopy for aberration-free imaging through intact mouse skull
a Conventional OCM image under an intact mouse skull before aberration correction. The thickness of the skull was about 85 μm, and the focal plane was set to a depth z0 = 125 μm from the upper surface of the skull. b MIP of five LS-RMM images taken over a depth range of 117–133 μm with 4-μm steps. LS-RMM image for each depth was obtained by stitching aberration-corrected 15 × 15 subregions. Note that myelinated fibers appear discontinuous in the image mainly due to coarse depth steps and large inclination angles of the fibers with respect to the image plane. c Aberration maps of subregions at z0 = 125 μm indicated by the gray dotted boxes in b. The size of the subregion is 10 × 10 μm2, and each phase map contains 9880 angular modes. Color bar, phase in radians. d MIP of TPF images at the same position as a before hardware aberration correction. The MIP image was obtained for a depth range of 119–135 μm with a 1.5-μm increment. e TPF image after physical aberration correction for the subregion indicated by the yellow box in b. Yellow boxes in d and e correspond to the same yellow box area in b. f, g MIP of TPF images at the depth z1 = 113 ± 1.5 μm before and after aberration correction, respectively, for the area, indicated as a white dashed box in d. h, i Same as f and g, respectively, for the depth z2 = 122 ± 1.5 μm. Color bar, intensity normalized by the maximum intensity in i. Scale bars indicate 30 μm in a, b and d, and 10 μm in e–i.
