Fig. 6: Deletion of Ring1 attenuates the expression of Shh in the telencephalon.

a The RPKM (reads per kilobase of exon per million mapped reads) scores for Gli1, Gli2, Ptch1, and Ptch2 in the RNA-seq analysis of NPCs from Ring1B KO embryos shown in Fig. 4 were normalized by those for the corresponding control sample in each experiment. Data are means ± s.d., n = 3 experiments. b RT-qPCR analysis of relative Ptch1 mRNA abundance (normalized by the amount of Actb mRNA) in ventral NPCs of control or Ring1B KO mice at E11. Data are means ± s.d., averaged values for n = 4 litters (the number and individual values of each littermate are provided in Source Data File), two-tailed Student’s paired t test. c Coronal sections of the brain of control or Ring1B KO mice at E10 were subjected to in situ hybridization analysis of Shh mRNA. Scale bars, 200 μm. d The Shh mRNA⁺ perimeter length as a proportion of total perimeter length for the telencephalic wall determined from sections similar to those in (c). Data are means ± s.d., n = 3 embryos of each genotype, two-tailed Student’s unpaired t test. e Coronal sections of the brain of Ring1A KO or Ring1A/B dKO mice at E10 were subjected to in situ hybridization analysis of Shh mRNA. Scale bars, 200 μm. Source data are provided as a Source Data file.