Fig. 5: PrimPol promotes RAD51 foci formation specifically in response to bulky adducts.
a Strategy for the S1-modified DNA fiber assay with ultraviolet (UV-C) irradiation. b CldU/IdU ratios after S1-modified DNA fiber assay in cells treated with 5 J/m2 UV-C or mock-treated as in (a), in the presence of nonT or PrimPol siRNA. Lines represent mean. Data from 4 repeats. c Percentages of cells with >5 RAD51 foci after release from 5 J/m2 UV, in presence of nonT or PrimPol siRNA. Release from mock-irradiation was 3 h. n = 3. d Percentages of cells with >5 RAD51 foci after treatment with 50 nM 4-nitroquinolie 1-oxide (4-NQO), in presence of nonT or PrimPol siRNA. The release from DMSO was 1 h. n = 3. e Percentages of cells with >5 RAD51 foci after treatment with 2 mM hydroxyurea (HU) for 24 h, in presence of nonT or PrimPol siRNA. n = 3. f Percentages of EdU positive cells with >10 RAD51 foci after release from 2 Gy ionizing radiation (IR), in the presence of nonT or PrimPol siRNA. Release from mock-irradiation was 2 h. n = 3. Source data are provided as a Source Data file. The means and SEM (bars) of at least three independent experiments are shown. Asterisks indicate p-values (One-way ANOVA for B; one-sided student’s t-test for C-F, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
