Fig. 3: OCTFs reveal an enhancer-promoter binding switch during exposure to genotoxic stimuli. | Nature Communications

Fig. 3: OCTFs reveal an enhancer-promoter binding switch during exposure to genotoxic stimuli.

From: Proteomic profiling and genome-wide mapping of O-GlcNAc chromatin-associated proteins reveal an O-GlcNAc-regulated genotoxic stress response

Fig. 3

a MCF-7 and ADR COGC-seq peak annotation relative to known genomic elements. b Heat map representation of the COGC-seq signal at the promoter and intron/intergenic regions bound by OCPs in MCF-7 and ADR cells. Enrichment levels (red, low; blue, high) were profiled ±3 kb from the peak center. c COGC-seq occupancy signal in MCF-7-biased, ADR-biased and unbiased peaks identified by MAnorm (fold change ≥ 2 and P value ≤ 10−5). Upper panel: Venn diagram showing the overlap of COGC-seq peaks in MCF-7 and ADR cells. The percentage of peaks annotated to promoter regions is indicated. Lower panel: Heat map representation of COGC-seq signal enrichment (red, low; blue, high) at differential quantitative peaks. The enrichment levels were profiled ±3 kb from the peak center. d Average enrichment profiles of published H3K27ac, H3K4me3 (GSE97481), H3K27me3 (GSE96363) and H3K4me1 (GSE86714) ChIP-seq reads at differential quantitative COGC-seq peaks. e, f Overlap of differential quantitative COGC-seq peaks with MCF-7 enhancer and super-enhancer regions reported previously. g, h Average enrichment profiles of COGC-seq reads at MCF-7 enhancer and super-enhancer regions.

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