Fig. 5: Metaproteomic detection of CE2/CE17-containing mannan PULs encoded in Roseburia- (MAG041) and Faecalibacterium- (MAG243) affiliated MAGs in pigs fed with either the control or 4% AcGGM diet.
From: Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut
Predicted gene organization and annotated gene function is color-coded and largely derived from the previous biochemical and structural characterization of the mannan degradation cluster (characterized genes indicated with *) in R. intestinalis L1-825. Gene synteny and identity % between mannan PULs found in R. intestinalis L1-82, MAG041 and MAG243 are indicated in gray boxes. Heat maps above detected enzymes show the LFQ detection levels for the four replicates sampled in control and 4% AcGGM-fed pigs. LFQ values of proteins from both clusters are in Supplementary Data 6. The predicted multi-modular mannanase (CBM27-GH26-CBM23) from MAG041 was the only extracellular protein in the locus, and the only extracellular mannanase expressed in response to AcGGM inclusion.
