Fig. 4: Transcriptional regulation of rice genes targeted by MoHTR1 and MoHTR2. | Nature Communications

Fig. 4: Transcriptional regulation of rice genes targeted by MoHTR1 and MoHTR2.

From: Two nuclear effectors of the rice blast fungus modulate host immunity via transcriptional reprogramming

Fig. 4

a Features of the constructs used to determine how MoHTR1 and MoHTR2 affect the expression of LUC under each rice gene promoter tested in rice protoplasts. MoHTR1-Δsp and MoHTR2-Δsp were expressed using the CaMV 35S promoter and the translation enhance sequence Ω. b Relative LUC activities in protoplasts transfected with MoHTR1-Δsp and MoHTR2-Δsp were compared with those transfected with the empty vector (EV). n = 6 independently transfected protoplasts; mean ± SD; *P < 0.01, two-sided Student’s t-tests. Three biological replicates produced similar results. c Expression patterns of the selected target genes in rice individually infected with ΔMoHTR1 and ΔMoHTR2 were compared with those in rice infected with KJ201. Mean ± SD, three independent experiments; *P < 0.01, two-sided Student’s t-tests. d Expression levels of individual target genes in MoHTR1-OX and MoHTR2-OX were compared with those in Nakdong (wild type) using qRT-PCR. Mean ± SD, three independent experiments; *P < 0.05, one-way analysis of variance (ANOVA) with Tukey’s honest significant difference (HSD) test. Detailed information about biological repeat experiments and statistical analysis underlying Fig. 4b–d are described as a Source Data file.

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