Fig. 5: IRF3 binds the ARM domain of β-catenin and prevents its nucleus translocation.
From: IRF3 prevents colorectal tumorigenesis via inhibiting the nuclear translocation of β-catenin
a, b Nucleocytoplasmic separation and immunoblot analysis of Active-β-catenin in HCT116 (a) and H1299 (b) cells after treated with wnt3a-conditioned medium. c Immunoblot analysis of the endogenous interaction between active-β-catenin, β-catenin, or GSK3β and IRF3 with anti-IRF3 immunoprecipitates in HCT116 cell line extracts after treated with wnt3a-conditioned medium. d Immunoblot analysis of the interaction between β-catenin or β-catenin-S33A and IRF3 with anti-FLAG immunoprecipitates in HEK293T cell line. e Pull-down analysis the interaction between GST-β-catenin, GST-β-catenin-ARM, or GST-β-catenin-Δ634-663 and MBP-IRF3. f, g Immunofluorescence (f) and nucleocytoplasmic separation (g) analysis for the cellular localization of β-catenin or its mutants in HEK293 cell line upon wnt3a-conditioned medium treatment. Red scale bars, 10 μm. Data represent three independent experiments (a–g). Source data are provided as a Source data file. See also Supplementary Fig. S5.
