Fig. 3: Functional profiles of different microbial groups and metabolites identified at different depths.

a Occurrence of core metabolic genes or pathways in phylogenetic clusters of MAGs. Phyla with only one representative MAG in the data set (n = 10) and MAGs that could not be classified (n = 10) are grouped together in the right-most column (Others). Dark and light color shading indicate gene presence in >50% and 1–50% of the genomes in each phylogenetic cluster, respectively. Complete lists of metabolic genes or pathways can be found in Supplementary Data 5. Detailed gene lists for each pathway indicated can be found at: https://github.com/bjtully/BioData/blob/master/KEGGDecoder/KOALA_definitions.txt. b Metabolites identified in sediment porewater from down core sediment subsamples. Metabolite levels were measured using HPLC Orbitrap mass spectrometry and are expressed as cumulative sum logarithmically normalized peak areas of triplicates. Alkyl-/arylalkylsuccinates and ethyl-CoM are signature metabolites for anaerobic hydrocarbon degradation, whereas other compounds shown can be derived from multiple biological or abiotic processes.