Fig. 1: Adult beta cells exhibit enhanced replication when transplanted into pre-weaning mice.
From: Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells
a–c Beta cell replication of 20wo mouse islet grafts 12-days after implantation into the anterior chamber of the eye of p16 or 20wo C57BL6/J recipients. a Representative images of islet grafts co-immunostained for insulin (purple) /ki67 (green) or insulin (purple)/pHH3 (green). Nuclei are marked with Hoechst in blue. b Quantification of the percentage of beta (insulin+) cells that are ki67+ in islet grafts transplanted into p16 (n = 11, blue) and 20wo (n = 5, purple) mice. c Quantification of the percentage of beta (insulin+) cells that are pHH3+ in islet grafts transplanted into p16 (n = 4, blue) and 20wo (n = 3, purple) mice. d–f Beta cell replication of adult human islet grafts 12-days after implantation into the anterior chamber of the eye of p16 or 20wo NSG-SCID mouse recipients (islets from two donors). d Representative images of islet grafts co-immunostained for insulin (purple)/ki67 (green) or insulin (purple)/pHH3 (green). Nuclei are marked with Hoechst in blue. e, f Quantification of the percentage of beta (insulin+) cells that are ki67+ (e) or pHH3+ (f) in human islet grafts transplanted into p16 (n = 5, green) or 20wo (n = 4, yellow) mice. Data shown represent mean ± SEM for the indicated n. *p < 0.05; **p < 0.01, using two-tailed Student’s t test. Scale bars are 25 μm.
