Fig. 5: Activation of Src–lipin-1 axis in human breast cancers and its correlation with clinical and pathological parameters.
From: Proto-oncogene Src links lipogenesis via lipin-1 to breast cancer malignancy
a–d Immunoblot analysis of p-Tyr795-lipin-1 and p-Tyr416-Src in breast tumour specimens and matched adjacent normal tissues. N tumour-adjacent normal tissue, T tumour, P1 patient 1. e The correlation between p-Tyr795-lipin-1 and p-Tyr416-Src in breast cancer. Ratios of p-Tyr795-lipin-1 to total lipin-1 and that of p-Tyr416-Src to total Src were calculated. f, g Increased p-Tyr795-lipin-1(f) and p-Tyr416-Src (g) in human breast tumours compared to tumour-adjacent normal tissue. Ratios of phosphorylated protein to total protein were calculated. h The correlation between the tumour size and p-Tyr795-lipin-1 in breast cancer patients. Ratios of p-Tyr795-lipin-1 to total lipin-1 was calculated. i, j The correlation between overall survival (i) or time to recurrence (j) and p-Tyr795-lipin-1 in breast cancer patients. P-Tyr795-lipin-1 level was assessed by immunoreactive score (IRS) of immunohistochemistry. k, l Kaplan–Meier survival curve showing overall survival or relapse-free survival of breast cancer patients is reduced in p-Tyr795-lipin-1-high group. P-Tyr795-lipin-1 level was assessed by IRS of immunohistochemistry and separated into higher half and lower half by median. f, g Data are median with interquartile range, two-tailed Mann–Whitney test; e, h, i, j Pearson’s correlation; k, l analysed using log-rank (Mantel–Cox) test. ***P < 0.001. Source data are provided as a Source Data file.
