Table 1 Kinetic parameters for prephenate dehydratase activity of CDT variants related to this studya.

From: Altered conformational sampling along an evolutionary trajectory changes the catalytic activity of an enzyme

Protein

KM (μM)

kcat (s−1)

kcat/KM (M−1 s−1)

AncCDT-140

ND

ND

ND

AncCDT-3/P18840

1830 ± 190

(1.04 ± 0.07) × 10−2

5.67 ± 0.70

AncCDT-3/L18840

294 ± 27

(4.58 ± 0.30) × 10−2

155 ± 18

AncCDT-5

277 ± 4

4.1 ± 0.2

(1.5 ± 0.1) × 104

PaCDT40

18.7 ± 2.9

18.4 ± 0.7

(9.8 ± 1.6) × 105

  1. ND not detected.
  2. aThe results are reported as mean ± standard error for KM and kcat. Errors for kcat/KM were obtained by propagation. Source data are provided as a Source Data file.
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