Fig. 1: Faecal microbiota of COPD patients (nā=ā28) can be distinguished from that of healthy individuals (nā=ā29) using 16S rRNA gene amplicon sequencing.

a Principal component (PC) analysis undertaken at the sequence variant level using read counts transformed using log-cumulative-sum scaling. b Multivariate sparse partial least-squares discriminant analysis (sPLS-DA) of read counts transformed using log-cumulative-sum scaling at the sequence variant level. c Sequence variants contributing to separation along with component 1 of sPLS-DA from b. Bar length indicates loading coefficient weight of selected genomes, ranked by importance, bottom to top; bar colour indicates the group in which the sequence variant has the highest median abundance, redā=āCOPD, blueā=āhealthy. d Heatmap of read counts transformed using log-cumulative-sum scaling of discriminatory sequence variants identified along with component 1 of sPLS-DA from (b).