Fig. 1: Landscape of DNA accessibility in 8 cell types from normal skin in vivo. | Nature Communications

Fig. 1: Landscape of DNA accessibility in 8 cell types from normal skin in vivo.

From: Chromatin accessibility landscapes of skin cells in systemic sclerosis nominate dendritic cells in disease pathogenesis

Fig. 1

(a) A schematic outline of the study design depicting the workflow for the isolation and ATAC-seq of 8 cell types (CD4+, CD8+ T cells (CD4, CD8), dendritic cells (DC), Langerhans cells (LC), endotheliocytes (EC), macrophages (Mac), fibroblasts (Fib), and keratinocytes (KC)) from healthy individuals (normal skin) and SSc patients (unaffected skin and affected skin). (b) Normalized ATAC-seq signal profiles at a locus in CD4, CD8, DC, LC, EC, Mac, Fib, and KC from healthy donors, shown together with a normalized H2K27ac chromatin immunoprecipitation sequencing profile. N represents the number of biological replicates. (c) Unsupervised hierarchical clustering of the Pearson correlations between all the samples. ATAC-seq signals were obtained from distal elements. Each row and each column is a sample, and cell types distinguished colors. Source data are provided as a Source Data file.

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