Fig. 1: Myeloid KLF2 regulates a macrophage-enriched metabolic network and is responsive to metaflammatory stress.

a Gene-set enrichment analysis of bone marrow-derived macrophages (BMDMs) from myeloid KLF2 knockout or control mice demonstrating significant enrichment in the macrophage-enriched metabolic syndrome network (MEMN) in addition to several innate immune pathways. Several annotated genesets from the Molecular Signature Database were explored including CGP chemical genetic perturbations, GO BP Gene Ontology biological processes, and KEGG Kyoto Encyclopedia of Genes and Genomes n = 4 biological replicates. b Heatmap of hierarchal clustering of MEMN genes demonstrating genotype clustering, based on DEGs from n = 4 biological replicates. c Gene ontology analysis demonstrating up and downregulated genesets for MEMN differentially expressed genes, based on DEGs from n = 4 biological replicates. d Klf2 expression from adipose tissue macrophages (ATM) harvested from WT mice fed either control diet (CD, n = 5) or high-fat diet (HFD, n = 4) for one month, n represent biologically independent mice, p = 0.0159. e KLF2 expression in human peripheral blood mononuclear cells (PBMC) from lean and obese patients, n = 10 biologically independent human subjects, p = 0.0068 f WT BMDM Klf2 expression after acute treatment with palmitic acid (PA), n = 5 biologically independent mice, p = 0.0079. *p < 0.05, **p < 0.01 by unpaired, two-tailed Student’s t-test, comparisons marked or indicated in figure legend. Error bars represent SEM. Source data are provided as a Source Data file.