Fig. 2: K2KO mice exhibit significant metabolic disease at baseline and with HFD.

a K2KO mice on control diet (CD) gain weight more rapidly than controls, n = 10, 2 weeks p = 0.0017, 3 and 4 weeks p < 0.0001. b K2KO mice have increased daily food intake in basal conditions, LysM n = 11, K2KO n = 10, p < 0.0001. c High fat diet (HFD) feeding exaggerates the obesity phenotype seen in K2KO mice, LysM n = 10, K2KO n = 12, 2 weeks p = 0.0083, 3 weeks p = 0.0012, 4 weeks p = 0.0133, 8 weeks p = 0.0038. d Increased energy intake in HFD-fed K2KO mice as measured by daily food consumption, n = 10, p < 0.0001. e Increased plasma leptin levels in HFD-fed K2KO mice indicative of increased adiposity and leptin resistance, n = 5, p = 0.0079. f Intraperitoneal insulin tolerance test (IPITT) demonstrates relative insulin resistance in HFD-fed K2KO mice, representative graph of n = 4, validated >3 times. AUC, area under curve, p = 0.0286. g Intraperitoneal glucose tolerance test (IPGTT) reveals relative glucose intolerance in HFD-fed K2KO mice, n = 4, validated >3 times, p = 0.0009. h Liver weight to body weight (BW) ratio demonstrating hepatomegaly in HFD-fed K2KO mice, n = 5, p = 0.0317. i Oil red O (ORO) staining indicating increased lipid accumulation in the livers of K2KO mice fed HFD. Quantification is of proportional area of ORO+ staining, representative images shown, LysM n = 5, K2KO n = 4, p = 0.0456. Scale bar = 100 µm j Respiratory exchange ratio (RER) from metabolic cage experiments over multiple light-dark cycles, mice were fed one month HFD, n = 4. *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired, two-tailed Student’s t-test, comparisons marked or indicated in figure legend. Error bars represent SEM. All mice on were fed CD/HFD for 1 month unless otherwise indicated. Metabolic parameters were also conducted at one month HFD unless otherwise indicated. n represents biologically independent mice throughout entire figure. Source data are provided as a Source Data file.