Fig. 4: Loss of myeloid KLF2 affects centrally-governed food intake and metabolic disease.
From: Myeloid Krüppel-like factor 2 is a critical regulator of metabolic inflammation
a Schematic describing pair-feeding protocol. b Pair-feeding K2KO mice (K2KO+PFHFD) abrogates weight gain compared to K2KO mice fed HFD ad libitum, LysM and K2KO ad lib. n = 4, K2KO PFHFD n = 7, ****p < 0.0001 comparing K2KO PFHFD to K2KO HFD by repeated measures one-way ANOVA with Tukey’s multiple comparison test. c Pair-feeding attenuates K2KO hepatomegaly, LysM n = 4, K2KO PFHFD n = 7. d Intraperitoneal glucose tolerance test (IPGTT) demonstrates improved glucose sensitivity in one month K2KO PFHFD mice, LysM and K2KO ad lib. n = 4, K2KO PFHFD n = 7, ****p < 0.0001 comparing K2KO PFHFD to K2KO HFD by repeated measures one-way ANOVA with Tukey’s multiple comparison test. e Intraperitoneal insulin tolerance test (IPITT) demonstrates improved insulin sensitivity in one month K2KO PFHFD mice, LysM and K2KO ad lib. n = 4, K2KO PFHFD n = 7, ****p < 0.0001 comparing K2KO PFHFD to K2KO HFD by repeated measures one-way ANOVA with Tukey’s multiple comparison test. f Pair-feeding K2KO mice rescues inflammatory gene transcription in WAT and liver, LysM and K2KO ad lib. n = 4, K2KO PFHFD n = 7. g Microglial ablated K2KO mice on HFD (using PLX5622 formulated HFD, PLX HFD) demonstrate improved weight gain over 8 weeks, LysM n = 7, K2KO+HFD and K2KO+PLXHFD n = 12, 7 weeks p = 0.0153, 8 weeks p = 0.0015 comparing K2KO+HFD to K2KO+PLX HFD. h Two months K2KO PLX HFD-fed mice consume less food than K2KO HFD mice, n = 4, p = 0.0284. i IPGTT demonstrates rescued glucose tolerance in K2KO PLX HFD mice, n = 12, p = 0.0042 at 15 min. j IPITT demonstrates rescued insulin sensitivity in K2KO PLX HFD mice, n = 12, p = 0.0482 (15 min), 0.0371 (30 min), 0.0003 (60 min), <0.0001 (90 min), 0.0001 (120 min) by unpaired two-tailed Student’s t-test, comparisons marked or indicated in figure legend. Error bars represent SEM. Pair-feeding experiments and associated metabolic parameters were performed for/at 1 month. PLX HFD metabolic parameters were performed at 1 month unless otherwise indicated. n represents biologically independent mice throughout entire figure. Source data are provided as a Source Data file.
