Fig. 6: Determination of M1- and M2-like phenotypes in macrophages in aortic tissues of WT and Ccl3^−/− mice after CaCl₂ treatment.

a, b Intra-aortic gene expression of a Nos2 (Pre: n = 4 in WT, n = 6 in Ccl3^−/−; 1 week- n = 4 in WT, n = 5 in Ccl3^−/−) and b Cd206 (Pre: n = 4 in WT, n = 6 in Ccl3^−/−; 1 week- n = 4 each in WT and Ccl3^−/−). **P < 0.01; *P < 0.05, vs. WT mice. c Triple-color immunofluorescence analysis of F4/80⁺CCR5⁺NOS2⁺ M1 macrophages and F4^/80⁺CCR5⁺CD206⁺ M2 macrophages in the AAA tissues of WT and Ccl3^−/− mice after CaCl₂ application. Representative images from five independent experiments are shown. Scale bar, 50 µm. d The ratio of M1 to M2 macrophages in WT and Ccl3^−/− mice (n = 5 each in WT and Ccl3^−/−). **P < 0.01, vs. WT. e The percentage of CCR5⁺ cells in M1 or M2 macrophages from WT and Ccl3^−/− mice after CaCl₂ application (n = 5 each). **P < 0.01, vs. WT. Unpaired two-sided Student’s t test was used in (a), (b), (d), and (e). Data are presented as mean values ± SEM.