Fig. 1: Msi2 marks leukemia stem cells in a mouse model of bcCML.

a Representative FACS plot shows GFP expression in Msi2-reporter bcCML. b Average frequency of GFP⁻negative (GFP⁻) and GFP-positive (GFP⁺) leukemic spleen cells ***P = 0.0005 (n = 4 mice). c Representative histograms show lineage expression in GFP⁻ and GFP⁺ leukemic spleen cells. d The average frequency of lineage-negative (Lin⁻) and lineage-positive (Lin⁺) cells within either the GFP⁻ or GFP⁺ fraction ****P < 0.0001 (n = 4 mice per group). e Number of colonies generated from GFP⁻ and GFP⁺ bcCML cells. **P = 0.002 (n = 3 technical replicates). f Representative FACS plot shows GFP expression within the lineage-negative (Lin⁻) fraction of the spleen from a leukemic mouse. g Number of colonies generated from Lin⁻ GFP⁻ and Lin⁻ GFP⁺ bcCML cells after primary and secondary plating. ***P = 0.0001 (n = 3 technical replicates each). h Schematic illustrates an experimental approach to test the ability of established GFP⁺ and GFP⁻ bcCML cells to drive disease development in secondary recipient mice. GFP⁺ or GFP⁻ cells from established bcCML were transplanted into recipients and i survival was monitored (n = 8 for GFP⁺ and n = 10 for GFP⁻). j The representative histogram shows GFP expression in leukemic spleen cells from a mouse with MLL-AF9/NRAS^G12V-driven AML (left panel); frequency of GFP⁻ and GFP⁺ leukemic spleen cells from mice with MLL-AF9/NRAS^G12V-driven AML ****P < 0.0001 (right panel; n = 3 mice per group). k Survival of mice transplanted with GFP⁻ and GFP⁺ MLL-AF9/NRAS^G12V-driven AML (n = 6 mice per cohort). l The representative histogram shows GFP expression in leukemic spleen cells from a mouse with BCR-ABL-driven CML (left panel); frequency of GFP⁻ and GFP⁺ leukemic spleen cells from mice with BCR-ABL-driven CML ****P < 0.0001 (middle panel, n = 4 mice per group); colony formation of Msi2-reporter⁺ and Msi2-reporter⁻ CML ****P < 0.0001 (right panel; n = 3 mice). m The representative histogram shows GFP expression in leukemic spleen cells from a mouse with NPM1-driven MPD (left panel); frequency of GFP⁻ and GFP⁺ leukemic spleen cells from mice with NPM1-driven MPD ****P < 0.0001 (middle panel, n = 3 mice per group); colony formation of Msi2-reporter⁺ and Msi2-reporter⁻ MPD ***P = 0.0003 (right panel; n = 3 mice per group). Two-tailed unpaired Student’s t-tests were used to determine statistical significance. Source data are provided as a Source Data File.