Fig. 7: Syndecan-1 mediates cell migration. | Nature Communications

Fig. 7: Syndecan-1 mediates cell migration.

From: A stem cell reporter based platform to identify and target drug resistant stem cells in myeloid leukemia

Fig. 7

a Schematic of transwell migration assay across either FN or FN+ HUVEC monolayer. b SDF-1 induced migration of shCTRL or shSdc1 bcCML cells across fibronectin-coated transwell filters. ***P = 0.0003 (n = 3 technical replicates, representative of four biological replicates). c SDF-1 induced migration of shCTRL or shSdc1 bcCML cells across HUVEC monolayers seeded on fibronectin-coated transwell filters. ****P < 0.0001 (n = 4 technical replicates, representative of two biological replicates). d Representative immunofluorescence of shCTRL or shSdc1 bcCML after 4 h incubation on fibronectin-coated transwell filters; green = shRNA vector, blue = DAPI. Quantification of polarized and non-polarized shCTRL or shSdc1 cells. *P = 0.01 (n = 3 biological replicates, >30 cells per condition). e–g Characterization of bcCML behavior when interacting with HUVECs on fibronectin-coated polyacrylamide gels (8 kPa). e Cell behaviors are stratified as: completely immobile (gray = static); immobile, but sensing its surroundings by extending lamellipodia and or filopodia (black = lamellipodia/filiopodia projecting); migratory, but not interacting with HUVECs (dark green = migratory); migratory and sustaining prolonged interactions with HUVECs (light green = interacting with HUVECs) (n > 10 cells per field of view for four fields across two independent experiments). f Representative time-lapse images demonstrating bcCML cell behavior in proximity to HUVECs on fibronectin-coated polyacrylamide gels (See also Supplementary Movies 4 and 5). g Duration of interactions between bcCML and HUVECs. (n = 33 events for shCTRL and n = 13 events for shSdc1). h SDF-1 induced migration of Sdc1−/− bcCML cells with empty control or wild-type Sdc1 ectopically expressed across HUVEC monolayers seeded on fibronectin-coated transwell filters. ****P < 0.0001 (n = 4 technical replicates). Two-tailed unpaired Student’s t-tests were used to determine statistical significance. Source data are provided as a Source Data File.

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