Fig. 3: Interaction of TDP-43 variants and Aβ. | Nature Communications

Fig. 3: Interaction of TDP-43 variants and Aβ.

From: TDP-43 interacts with amyloid-β, inhibits fibrillization, and worsens pathology in a model of Alzheimer’s disease

Fig. 3

a Interaction of Aβ with TDP-43 variants was determined by ELISA. TDP-43 proteins were coated and detected by different concentrations of biotinylated Aβ. The averaged data from three replicates and standard deviations are plotted (TDP-43_FL, red; TDP-43_265, purple; TDP-43_N-term, green; TDP-43_RRM1 + 2, blue). b–e Binding response between Aβ40 and TDP-43 variants in biolayer interferometry analysis. TDP-43 at different concentrations (μM) were prepared and subjected to biolayer interferometry analysis by using Octet RED96 System (Pall ForeBio). The real-time binding response (nm) was measured in seconds. The loading amounts of Aβ40 are the same for all the experiments. Source data are provided as a Source Data file.

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