Fig. 1: Widespread BHV infection across the bat phylogeny.

The bat phylogeny (left) was extracted from the mammalian super-tree to contain species that were tested here or elsewhere for BHV infection^30,71. Labels on tree branches indicate bat families. Taxa in black text indicate bat species tested here for the first time. Stacked bar charts (right) indicate the number of saliva samples that tested either positive or negative for BHV by PCR of the terminase gene UL89. A binomial generalised linear mixed model (GLMM) followed by post-hoc pairwise comparisons of estimated prevalence by species (multiple comparisons of means: Tukey contrasts, two tailed) was used to test the significance of differences in prevalence. The pairwise significance of prevalence in each species compared to D. rotundus is shown on the bar chart with corrected P-values: > 0.1, * > 0.05, ** > 0.01, *** > 0.005. All other pairwise comparisons were non-significant. D. rotundus is indicated with a red arrow. Source data are provided as a Source Data file.