Fig. 1: BRD4 protein levels are higher in DMD and mdx muscles. | Nature Communications

Fig. 1: BRD4 protein levels are higher in DMD and mdx muscles.

From: BETs inhibition attenuates oxidative stress and preserves muscle integrity in Duchenne muscular dystrophy

Fig. 1

a Representative images for immunoblot of BRD2, BRD3, and BRD4 in WT and mdx mice. Vinculin is used as a loading control. Right panel, signal quantification was performed with ImageJ. Data are expressed as the mean ± SD, n = 3, **denotes P < 0.01 was determined by using unpaired two-sided t-test. a indicates statistical significance compared to the control group. b RNA of control and mdx mice was analyzed for BRD2 (WT, n = 4; mdx, n = 4), BRD3 (WT, n = 4; mdx, n = 4) and BRD4 (WT, n = 7; mdx, n = 5) levels by qRT-PCR. Data were normalized against HPRT. Data are expressed as the mean ± SD, statistical significance was calculated using unpaired two-sided t-test. c Immunoblot analysis of paravertebral muscle specimen from Duchenne patients (n = 4) and healthy controls (n = 2). Normalized band intensity in immunoblots is reported above signals. d RPKM expression levels of BRD4 transcript in previously reported RNA-Seq dataset for DMD (n = 6) and healthy donors (n = 6). Data are expressed as the mean ± SD.

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