Fig. 7: Functional amelioration mediated by JQ1 persists following withdrawal.

a Treadmill (WT: n = 3; mdx veh: n ≥ 7; mdx JQ1: n ≥ 8), inverted screen (n = 9 for each experimental group) and wire (wt animals: n = 9; mdx mice: n = 18 for each group) tests were performed on control, vehicle- and mdx-treated mice. Data are expressed as the mean ± SEM). *P < 0.05, **P < 0.01 and ***P < 0.001 were determined by one-way ANOVA followed by Tukey’s post hoc test for the treadmill test and with Kruskal–Wallis test followed by Dunns post hoc for wire and inverted screen tests. a indicates statistical significance compared to Control group; b indicates statistical significance compared to the mdx mice animal group. b Mice were treated with JQ1 or vehicle for 3 weeks and treadmill (n = 3 for each experimental group), inverted screen (n = 9 for each group) and wire (n ≥ 10 for each group) tests were performed once a week, and for additional 3 weeks after JQ1 withdrawal. Data are expressed as the mean ± SEM. *P < 0.05 and **P < 0.01 indicate statistical significance versus mdx-vehicle group, and were determined by one-way ANOVA followed by Tukey’s post hoc test.