Fig. 4: Electron tomography of vesicles captured by golgin-97-mito.
From: Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers
a CLEM of a Lowicryl-embedded HeLa cell expressing golgin-97-mito and TMEM87A-RFP throughout a thickness of approximately 350 nm. Mitochondria are large dense structures (pink stars) sandwiching small circular structures. Images are representative of two independent experiments. Scale bars 100 nm. b–d Boxplots showing quantitation of spherical structures in a through 3D segmentation using LimeSeg (50 quantified structures over two independent tomograms, data provided in the Source Data file), boxplots as for Fig. 1g. e 3D projections of indicated quantified structures in b–d, scale in nm. Data are provided as a Source Data file.
