Fig. 6: GCC88 and golgin-97/golgin-245 have redundant roles that together support cell viability.

a Immunoblot of proteins from wild-type and mutant HeLa cells probed with antibodies against the indicated proteins, blot representative of two independent experiments. b Confocal micrographs of wild-type and mutant HeLa cells stained for endogenous TMEM87A (cargo), GM130 (marker of cis-Golgi) and DAPI. Micrographs representative of three independent experiments, scale bars, 10 μm (5 μm in zoom). c Quantification of b measuring the ratio of the intensity of TMEM87A at the Golgi over that of GM130. Boxplots are of data from three independent replicates, n ≥ 300 per replicate, as for Fig. 1g. P-values are of two-sided two-sample t-tests comparing the means of ratios from the three replicates for each cell type. d Effect of knockdown of GCC88 on growth of Δgolgin-97 / Δgolgin-245 double knockout HeLa cells - three replicates per time point (error bars show mean and SD). Data are provided as a Source Data file.