Fig. 2: MiCas9 increases KI rates and reduces on-target indel rates in ds-KI applications. | Nature Communications

Fig. 2: MiCas9 increases KI rates and reduces on-target indel rates in ds-KI applications.

From: MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

Fig. 2

a Percentage of GFP+ cells after GFP ds-KI to the AAVS1 locus in different cell types. b Percentage of GFP+ cells after GFP knock-in at the AAVS1 locus using different nucleases. c On-target indel rates at the AAVS1 locus after GFP ds-KI using different nucleases. AEC: airway epithelial cells, iPSCs: induced pluripotent stem cells, RNP: Cas9/gRNA ribonucleoprotein, Sp: spCas9, Mi: miCas9, HE: Cas9-HE, GE: Cas9-GE. #Reads: Average amplicon reads per sample. Three independent experiments were performed for each condition. Data are presented as mean ± standard error of means (SEM). Unpaired t-test (two tailed) was used to compare data using GraphPad Prism 8 software (GraphPad Software, Inc., San Diego, CA). Source data are available in the Source Data file.

Back to article page