Fig. 4: Effects of miCas9 on ss-PGE applications. | Nature Communications

Fig. 4: Effects of miCas9 on ss-PGE applications.

From: MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

Fig. 4

a PGE rates, on-target indel rates, and the noise/signal ratio at EGFR by spCas9 and miCas9 in human iPSCs. b PGE rates, on-target indel rates, and noise/signal ratio at B2M by spCas9 and miCas9 in human iPSCs. c PGE rates, on-target indel rates, and noise/signal ratio at HBB by different nucleases in human iPSCs. d PGE rates, on-target indel rates, and noise/signal ratio at CFTR-G542 by different nucleases in human iPSCs. Sp: spCas9, Mi: miCas9, HE: Cas9-HE, GE: Cas9-GE. #Reads: Average amplicon reads per sample. Three independent experiments were performed for each condition. Data are presented as mean ± standard error of means (SEM). Unpaired t-test (two tailed) was used to compare data using GraphPad Prism 8 software (GraphPad Software, Inc., San Diego, CA). Source data are available in the Source Data file.

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