Fig. 4: Entrainment of vasodilation and vasoconstriction of a 3D vasculature network with pupil diameter measured by Bessel TPLSM.
From: High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
a A 1.4 mm × 1.4 mm × 0.1 mm volume of vasculature imaged at 15 Hz with Gaussian TPLSM, color-coded by depth. b Bessel TPLSM image of the same volume in a, visualized in grayscale on the normalized square root of fluorescence signal. c Example pupil images at time points 1 and 2 of the ipsilateral eye acquired concurrently with vasculature imaging. Dashed yellow ovals: pupil profiles automatically segmented from video data. d Pupil diameter and signal time traces of two regions of interest (ROIs, arrows and black squares in e), showing positive and negative correlation with pupil diameter, respectively. CC: correlation coefficients. Orange circles on pupil time trace indicate time points 1 and 2. e Map of cross-correlation coefficients between ROIs tiling the FOV in b and pupil diameter. Representative data from four mice. Scale bars: 200 µm for a, b, and e; 1 mm for c. Post-objective power: Gaussian: 47 mW; Bessel: 167 mW.
