Fig. 4: ssRNA phage with two functional lysis genes.
From: Rapid de novo evolution of lysis genes in single-stranded RNA phages
a Lysis profiles of two sgl candidates (Beihai9_1 and Beihai9_2); pBAD24 (red open circle), Beihai9_1 (blue open squares), Beihai9_2 (green open diamond).The genomic context of sgls is shown relative to the rep gene (green bar) with the functional candidate (Beihai9_1) shown as dark blue and the non-functional candidate as light blue. b The lysis profiles of Beihai9_2 gain-of-function mutants 123 and 131; pBAD24 (red open circle), Beihai9_2 (blue open square), Beihai9_2 mut121 (light orange-filled triangle), Beihai9_2 mut123 (green-filled square), Beihai9_2 mut131 (black-filled right triangle). c Table summarizing the base and amino acid changes of the mutants in b. d The lysis profiles of Beihai9_2 of single and double mutant constructs; pBAD24 (red open circle), Beihai9_2 (blue open squares), Beihai9_2 mut123 (green open diamond), Beihai9_2 double mutant g15t A54D (black cross), Beihai9_2 double mutant g15t L51V (pink plus), Beihai9_2 g15t (light blue open triangle), Beihai9_2 L51V (light green-filled circle), and Beihai9_2 A54D (orange-filled squares). The lysis profiles shown here are representative of three biological replicates.
