Fig. 6: De novo gene evolution in ssRNA phages sourced from relatively close geographic locations.

a Geographic locations and the invertebrate animal sources for the transcriptomes that contained the genomes of Wenzhou1, Wenzhou6, Wengling2, and Sanxia1. Silhouette images were obtained from phylopic.org, courtesy of Christoph Schomburg (shrimp), Joanna Wolfe and T. Michael Keesey (pan-Crustacea), and Scott Hartman (snail) under CC0 1.0 (https://creativecommons.org/publicdomain/zero/1.0/). b Nucleotide dot plot between Sanxia1 and Wenzhou1 genomes. The gaps in the first ~3000 bases of the alignment are boxed and numbered. Bi-directional arrows indicate the shift in the location of the sgls. c Dot plot of the maturation protein primary structures of Sanxia1 and Wenzhou1. d Similar to b but Wenzhou6 vs Wenling2 comparison. e Model for de novo lysis gene evolution after changing pili specificity and presumably the host. f Sequence alignment of the region represented in box 4 of panel b with base changes highlighted red and indicated with red arrows. The indels are shown as orange loops with the size of the indel indicated above the loops. The translated reading frames are shown above (Wenzhou1) or below (Sanxia1) the nucleotide sequence and the amino acids shared between Sgl^Sanxia1 and Wenzhou1 reading frame are colored green.