Fig. 6: Performance of BCM3D on mixed population biofilm images.

a Cell counting accuracy of BCM3D on simulated images containing different ratios of rod-shaped and spherical cells. Black diamonds represent the counting accuracy for N = 10 independently simulated datasets. Green dots represent the cell density for each independent dataset. Error bars represent ± one standard deviation. b Cell counting accuracy of BCM3D on simulated images with different ratios of membrane-labeled, and membrane-labeled and interior fluorescent protein expressing cells. Black diamonds represent the counting accuracy for N = 10 independently simulated datasets. Green dots represent the cell density for N = 10 independent datasets. Error bars represent ± one standard deviation. c, d Cell counting accuracy of BCM3D on experimental images of c membrane-labeled, and d membrane-labeled and interior fluorescent protein expressing E. coli cells (mixing ratio ~1:1). Each data point is the average of the cell counting accuracies calculated using annotation maps traced by three different researchers (N = 3). Data are presented as mean values ± one standard deviation indicated by error bars. Source data are provided as a Source data file for Fig. 6.