Fig. 6: SLC25A51 is the functional ortholog of the yeast NAD+ transporter Ndt1p.

a Oxygen consumption rate (OCR) in wt and SLC25A51-deficient HAP1 cells reconstituted with mitochondrially localized GFP (mGFP) or the yeast NAD+ transporter NTD1. Results of three independent biological replicates are shown. The bars show average value across biological replicates. Statistical significance was calculated with a one-way ANOVA with Dunnett’s multiple comparison test. ns: nonsignificant, ***p-value < 0.01. Source data are provided as a Source Data file. b Fold change in mitochondrial NAD content in wt or SLC25A51-deficienent cells expressing mitoGFP or Ndt1p (mean ± SD, n = 2 technical replicates). Source data are provided as a Source Data file. c Effect of SLC25A51 on the growth of the Δndt1Δndt2 yeast strain. Yeast strains were grown in a YNB medium supplemented with 2% ethanol. The values of optical density at 600 nm refer to cell cultures after the indicated periods of growth. Data from a representative experiment are reported. Similar results were obtained in three independent experiments. d Effect of SLC25A51 on the mitochondrial NAD+ content of the Δndt1Δndt2 yeast strain. Mitochondrial extracts were assayed for NAD+ content by HPLC analysis. Data represent means ± SE of three independent experiments and were subjected to one-way analysis of variance followed by Tukey’s post hoc tests (**p-value < 0.05, *p-value < 0.1). EV: empty vector, pA51: SLC25A51 plasmid.