Fig. 8: IHC examination of tissue from K18 hACE2 transgenic and WT C57BL/6 mice infected with SARS-CoV-2.
From: Lethality of SARS-CoV-2 infection in K18 human angiotensin-converting enzyme 2 transgenic mice
a WT and K18 hACE2 transgenic C57BL/6 mice nasal turbinate (top, at 2 DPI), lung tissue (middle, at 2 DPI), and brain (bottom, at 4 DPI) stained with an antibody against SARS-CoV-2 NP (red) or with antibody recognizing hACE2 receptor (red). b Infected choroid plexus in the brains of SARS-CoV-2-infected K18 hACE2 transgenic mice at 4 DPI. Left panel show that the hACE2 receptor (red) is highly expressed in the choroid plexus (all nuclei shown in aqua-blue). Right panel shows that some cells in the choroid plexus are infected with SARS-CoV-2 as these are positive to a SARS-CoV-2 NP (in red). c Nasal turbinates, d lung, and e brain from SARS-CoV-2-infected K18 hACE2 transgenic and wild-type mice at 2 DPI (bottom) and 4 DPI (top). Representative images of n = 8 for K18 hACE2 transgenic mice (50:50 male:female, per time point) and n = 2 for WT mice (50:50 male:female, per time-point) randomly chosen. Images magnified ×10 and ×20. Further magnification of selected areas (left, magnification bar 20 μm). hACE2 receptor (red), SARS-CoV-2 NP (green) as observed in nasal turbinates, lung, and brain tissues of K18 hACE2 transgenic mice (Blue: DAPI). Colocalization of hACE2 and SARS-CoV-2 NP is shown in the merged images. Data are representative over two independent experiments.
