Table 3 T- and B-cell evaluation of SARS-CoV-2-infected K18 hACE2 transgenic C57BL/6 mice in lung and brain and severity scored.
From: Lethality of SARS-CoV-2 infection in K18 human angiotensin-converting enzyme 2 transgenic mice
T- and B-cell labeling in lung and brain | |
|---|---|
Day 2 | Lung: 6/6 had prominent CD3-positive round cells centered on and adjacent to bronchioles and surrounding vascular structures with lesser numbers extending into alveolar interstitium and alveolar spaces. 6/6 had rare intra-bronchiolar and intra-alveolar round cells with strong CD3 cytoplasmic staining. Semi-quantitative assessment of CD3-positive round cells correlates with the H&E inflammation scores; 5/6 had few/scant CD20-positive round cells with uniform distribution throughout alveolar septa and 1/6 had slightly increased CD20-positive round cells surrounding vascular structures. Brain: 3/6 with rare intra-parenchymal and perivascular CD3 immunostaining; 2/6 with single/sparse CD3-positive cells located intra-parenchymal, ependymal, interstitium, and perivascular; CD20 negative. 5/6 CD20 negative. |
Day 4 | Lung: 4/6 had diffuse and 2/6 regional/multifocal CD3-positive round cells within alveolar septa, vascular structures, and bronchiolar walls. 6/6 had individual intra-bronchiolar and intra-alveolar CD3-positive round cells with strong cytoplasmic staining. Semi-quantitative assessment of CD3-positive round cells correlates with the H&E inflammation scores; 4/6 CD20-positive round cells had few/scant round cells within the interstitium with a uniform distribution and 2/6 had slightly increased CD20 round cells within areas of interstitial inflammation. Brain: 5/6 Few or sparse CD3-positive cells located intra-parenchymal and perivascular or interstitium of ependyma and rimming vascular structures; 5/6 CD20 negative; 1/6 perivascular CD20-positive cells. |
Day 6 | Lung: 6/6 had prominent CD3-positive round cells within alveolar septa, intra-alveolar spaces and vascular walls. 4/6 had diffuse/uniform or 2/6 regional distribution of CD3-positive round cells throughout the interstitium with occasional small aggregates expanding the alveolar septa or vascular adventitia. 6/6 had rare intra-bronchiolar and intra-alveolar CD3-positive cells with strong cytoplasmic staining. Semi-quantitative assessment of CD3-positive round cells correlates with the H&E inflammation scores; 6/6 had few/scant CD20-positive round cells with uniform distribution throughout alveolar septa. Brain: 5/6 significant/mild-marked intra-parenchymal and perivascular CD3-positive cells: 3/6 with moderate to marked meningeal CD3-positive cells. 5/6 CD20 negative or rare. |
