Fig. 1: Hydrogel-evoked FBRs vary and exhibit cellular features of CNS wound response. | Nature Communications

Fig. 1: Hydrogel-evoked FBRs vary and exhibit cellular features of CNS wound response.

From: Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions

Fig. 1

a Schematic and chemical structures of synthetic hydrogels used as tools to study CNS FBR. b Experimental model of in vivo injections into caudate putamen (CP) of mouse forebrain. c, d Survey and detail images of stromal and inflammatory cells (CD13), astrocytes (GFAP), and neurons (NeuN) at 1 week after injections of PBS, hydrogels, or L-NIO-induced stroke. e–g Quantification of total immunohistochemical staining. e Total CD13 in CP. not significant (NS), ***P < 0.0001 versus PBS injection, one-way ANOVA with Bonferroni. f Total CD13 in either non-neural or neural tissue compartments. NS or ***P < 0.0001 versus PBS injection, two-way ANOVA with Bonferroni. g Total GFAP in CP. NS or ***P < 0.0001 versus DCHMO, one-way ANOVA with Bonferroni. All graphs are mean ± s.e.m with individual data points superimposed showing n = 10, 10, 12, and 4 mice per group for PBS, DCHMO, DCHK, and L-NIO, respectively. AU arbitrary units, G hydrogel, IC internal capsule.

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