Fig. 1: Phenotypic and functional heterogeneity of CAFs.

a t-SNE plot of single cell RNAseq data generated from 4T1 tumors. The cluster identified as CAFs is highlighted in teal. b Bubble plot outlining the expression of canonical lineage cell markers utilized for cell cluster annotation. c CAF-only t-SNE projection showing emergent sub-clusters. d Biplot of Principle Components 1 and 2 from analysis of genes with highest variability (s.d. >=5), each dot represents a CAF subset from one of five independent samples. e Pearson correlation analysis of CAF subsets from each sample. f Violin plots depicting log expression level of selected genes. g Pathway enrichment analysis was performed on genes differentially expressed by each CAF subset using gProfiler (log(FC) > 1, Reactome pathways with p-value <0.05 were selected for, Fisher’s one-tailed test was used to calculate p-values, and p-values were adjusted for multiple testing). n = 5 samples from 3 independent experiments.