Fig. 4: TGFβ-blockade ablates myofibroblasts and induces expansion of CD73⁺ CAFs.

a Superimposed t-SNE projections of CAF-curated single cell RNAseq data from isotype- and anti-TGFβ-treated mice. n = 4 mice per group from 2 independent experiments. b Representative t-SNE plots highlighting CAF clusters in isotype- and anti-TGFβ-treated mice. c Frequency of CAF subsets within the overall CAF cluster. Each column represents a mouse. n = 4 mice per group from 2 independent experiments. d Quantification of CAFs expressing αSMA from dissociated 4T1 tumors of isotype- and anti-TGFβ-treated mice by flow cytometric analysis. Each dot represents a mouse. Mean is depicted. n = 10 mice per group. Data are representative of four independent experiments. ****p < 0.0001 (unpaired, two-tailed t-test with Welch’s correction). e Violin plot depicting expression of Nt5e in ilCAFs. n = 4 mice per group from 2 independent experiments. f Representative flow cytometric analysis of CD73 expression on CAFs from dissociated 4T1 tumors from isotype- and anti-TGFβ-treated mice. g Quantification of CAFs expressing CD73 as a frequency of single cells from dissociated 4T1 tumors of isotype- and anti-TGFβ-treated mice by flow cytometric analysis. Each dot represents a mouse. Mean is depicted. n = 10 mice per group. Data are representative of four independent experiments. ****p < 0.0001 (unpaired, two-tailed t-test with Welch’s correction). h Pseudotime analysis overlaid on UMAP projection depicting divergent trajectories among the five CAF subsets. Each dot represents a cell, and each curve represents a lineage.