Fig. 5: ilCAFs exhibit an immunomodulatory transcriptomic signature. | Nature Communications

Fig. 5: ilCAFs exhibit an immunomodulatory transcriptomic signature.

From: TGFβ-blockade uncovers stromal plasticity in tumors by revealing the existence of a subset of interferon-licensed fibroblasts

Fig. 5

a Bubble plot outlining the expression of the indicated cell markers in the different CAF subsets. b Expression heatmap of the top 25 differentially expressed genes in ilCAFs. c Pathway enrichment analysis was performed on genes differentially expressed by ilCAFs using gProfiler (log(FC) > 1, Reactome pathways with p-value <0.05 were selected for, Fisher’s one-tailed test was used to calculate p-values, and p-values were adjusted for multiple testing). d Violin plots depicting expression of selected genes in CAF subsets. n = 4 mice per group from 2 independent experiments. e Flow cytometric analysis of MHCII expression by CAFs in isotype- and anti-TGFβ-treated mice. Each dot represents a mouse. Mean is depicted. n = 3 mice per group from one experiment. *p = 0.0189 (unpaired, two-tailed t-test with Welch’s correction). f The differentially expressed receptor-ligand pairs between CAFs and T cells are shown (p-value <0.05, calculated using Kruskal–Wallis test).

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